- Data Note
- Open Access
- Open Peer Review
Improved hybrid de novo genome assembly of domesticated apple (Malus x domestica)
- Xuewei Li†1,
- Ling Kui†2,
- Jing Zhang3,
- Yinpeng Xie1,
- Liping Wang1,
- Yan Yan1,
- Na Wang1,
- Jidi Xu1,
- Cuiying Li1,
- Wen Wang2,
- Steve van Nocker4,
- Yang Dong5, 6Email author,
- Fengwang Ma1Email author and
- Qingmei Guan1Email author
© The Author(s). 2016
- Received: 24 May 2016
- Accepted: 14 July 2016
- Published: 8 August 2016
Domesticated apple (Malus × domestica Borkh) is a popular temperate fruit with high nutrient levels and diverse flavors. In 2012, global apple production accounted for at least one tenth of all harvested fruits. A high-quality apple genome assembly is crucial for the selection and breeding of new cultivars. Currently, a single reference genome is available for apple, assembled from 16.9 × genome coverage short reads via Sanger and 454 sequencing technologies. Although a useful resource, this assembly covers only ~89 % of the non-repetitive portion of the genome, and has a relatively short (16.7 kb) contig N50 length. These downsides make it difficult to apply this reference in transcriptive or whole-genome re-sequencing analyses.
Here we present an improved hybrid de novo genomic assembly of apple (Golden Delicious), which was obtained from 76 Gb (~102 × genome coverage) Illumina HiSeq data and 21.7 Gb (~29 × genome coverage) PacBio data. The final draft genome is approximately 632.4 Mb, representing ~ 90 % of the estimated genome. The contig N50 size is 111,619 bp, representing a 7 fold improvement. Further annotation analyses predicted 53,922 protein-coding genes and 2,765 non-coding RNA genes.
The new apple genome assembly will serve as a valuable resource for investigating complex apple traits at the genomic level. It is not only suitable for genome editing and gene cloning, but also for RNA-seq and whole-genome re-sequencing studies.
- Malus x domestica
- Illumina sequencing
- PacBio sequencing
Whole-genome shotgun sequencing of ‘Golden Delicious’ apple on the Illumina platform
Genomic DNA was extracted from leaf tissues of a single ‘Golden Delicious’ apple tree with the GenElute™ Plant Genomic DNA Miniprep Kit (Sigma-Aldrich; St. Louis, USA). Paired-end libraries with insert sizes ranging from 350–500 bp were constructed with Next UltraTM DNA Library Prep Kit for Illumina (NEB; USA) according to the manufacturer’s instructions. These libraries were sequenced on an Illumina HiSeq 4000 platform (Illumina; CA, USA) using the PE-150 module , and yielded about 86 Gb of raw data. These data were then subjected to filtering to remove: (1) reads in which more than 5 % of bases were N or poly-A; (2) reads in which more than 30 bases were of low quality; (3) reads with adapter contamination; (4) reads shorter than 30 bp; and (5) PCR duplicates. These steps yielded a clean sequence of ~76 GB, representing about 102 × genome coverage (Additional file 1: Table S1). De novo assembly was performed with with SOAPec_v2.01  using default parameters.
Single-molecule long reads from the PacBio RS II platform (Pacific Biosciences, USA) were used to assist the subsequent de novo genome assembly . In brief, 15 μg of sheared DNA was used to construct five SMRT Bell libraries with an insert size of 17 kb. The libraries were then sequenced in 20 single-molecule real-time DNA sequencing cells using the P6 polymerase/C4 chemistry combination, and a data collection time of 240 min per cell. The sequencing produced about 21.7 Gb data, consisting of 2,759,937 reads with an average read length of 7,863 bp (Additional file 1: Figure S1). The polymerase read N50 length after single passing was around 16.6 kb, and the polymerase read quality was greater than 82.4 % (Additional file 1: Table S1).
Quality-filtered reads from the Illumina platform were subjected to 23-mer frequency distribution analysis with Jellyfish . Analysis parameters were set at -k 23, and the final result was plotted as a frequency graph (Additional file 1: Figure S2). Two distinctive modes were observed from the distribution curve: the higher peak at a depth of 88 reflected the high heterozygosity of the apple genome; the lower peak provided a peak depth of 179 for the estimation of its genome size. Based on the total number of k-mers (125,428,662,216), the apple genome size was calculated to be approximately 701 Mb, using the following formula: genome size = k-mer_Number/Peak_Depth.
A hybrid genome assembly pipeline was used to overcome challenges posed by heterozygous apple genome (Additional file 1: Figure S3). An Illumina-based de novo genome assembly was first generated using Platanus , yielding a total length of 1.05 Gb, with a contig N50 length of 534 bp. Then, all PacBio RS reads were used in the hybrid assembly process via the DBG2OLC  pipeline with the following parameters: LD10, MinLen 200, KmerCovTh 2, MinOverlap 10, AdaptiveTh 0.001, and RemoveChimera 1. This led to a preliminary apple genome assembly of 632.4 Mb with a contig N50 size of 111,619 bp, representing ~90 % of the estimated apple genome (701 Mb). The contig N50 size represents a ~6.9 fold improvement in length from the previously reported 16.1 kb . These improvements were made possible by introducing the long-read sequencing strategy (Additional file 1: Figure S4), which increased the sequencing precision of repeats.
Statistics of the completeness of the hybrid de novo assembly genome of ‘Golden Delicious’ based on 248 core eukaryotic genes, produced by the software CEGMA  with default parameters
Tandem Repeat Finder  was used to identify tandem repeats in the ‘Golden Delicious’ apple genome. RepeatMasker and RepeatProteinMasker  were used against Repbase  to identify known transposable element repeats. In addition, RepeatModeler  and LTR FINDER  were used to identify de novo evolved repeats. The combined results show that the total length of repeated sequences is about 382 Mb, accounting for ~60 % of the ‘Golden Delicious’ apple genome assembly (Additional file 1: Table S4).
Statistics for ‘Golden Delicious’ genome protein-coding sequences annotation
tRNAscan-SE (version 1.31)  software with default parameters for eukaryotes was used for tRNA annotation. rRNA annotation was based on homology with rRNAs from several diverse higher plant species (not shown), using BLASTN with ‘E-value = 1e-5’. miRNA and snRNA genes were predicted by INFERNAL software  using the Rfam database (release 11.0) . The final results included 321 miRNAs, 274 tRNAs, 605 rRNAs, and 480 snRNAs (Additional file 1: Table S5).
CDS, coding DNA sequence; NCBI, National Center for Biotechnology Information
This work was support by the National Science Foundation of China (31572106) and QG is supported by funding from the Thousand Talents Plan.
FM, QG, YD and WW designed the study. JZ assembled the genome. XL, YX and NW extracted DNA, LK constructed libraries, LW and YY analyzed the data. QG, JZ XL, and SN wrote the manuscript. All authors read and approved the final manuscript.
JZ is a shareholder of Agri-biotech Lab Company (Kunming, Yunnan).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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