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Data access for the 1,000 Plants (1KP) project
GigaScience volume 3, Article number: 17 (2014)
The 1,000 plants (1KP) project is an international multi-disciplinary consortium that has generated transcriptome data from over 1,000 plant species, with exemplars for all of the major lineages across the Viridiplantae (green plants) clade. Here, we describe how to access the data used in a phylogenomics analysis of the first 85 species, and how to visualize our gene and species trees. Users can develop computational pipelines to analyse these data, in conjunction with data of their own that they can upload. Computationally estimated protein-protein interactions and biochemical pathways can be visualized at another site. Finally, we comment on our future plans and how they fit within this scalable system for the dissemination, visualization, and analysis of large multi-species data sets.
The 1,000 plants (1KP) project is an international multi-disciplinary consortium that has now generated transcriptome data from over 1,000 plant species. One of the goals of our species selection process was to provide exemplars for all of the major lineages across the Viridiplantae (green plants), representing approximately one billion years of evolution, including flowering plants, conifers, ferns, mosses and streptophyte green algae. Whereas genomics has long strived for completeness within species (e.g., every gene in the species), we were focused on completeness across an evolutionary clade – obviously not every species, but one representative species for everything at some phylogenetic level (e.g., one species per family, and perhaps more than one species when the family is especially large). Because many of our species had never been subjected to large-scale sequencing, 2 gigabases (Gb) of data per sample was sufficient to increase the number of plant genes by approximately 100-fold in comparison to the totality of the public databases.
The 1KP project began as a public-private partnership, with 75% of the funding provided by the Government of Alberta and 25% by Musea Ventures. Significant in-kind contributions were provided by BGI-Shenzhen in the form of reduced sequencing costs and by the NSF-funded iPlant collaborative  in the form of computational informatics support. Many plant scientists from around the world were involved in the collection of live tissue samples and in the extraction of RNA. Additional computing resources were provided by Compute Canada and by the China National GeneBank. Despite the constraints of this funding model, we released our data (on a collaborative basis) to scientists who approached us with goals that did not compete with ours. For the general community, access was provided through a BLAST portal .
We believed that there would be intrinsic value in data of this nature that is beyond our imagination. But for the initial publication, we agreed on two objectives. Firstly, by adopting a phylogenomics approach we hoped to resolve many of the lingering uncertainties in species relationships, especially in the early lineages of streptophyte green algae and land plants, where previous analyses were based on comparatively sparse taxonomic densities. And secondly, despite the limitations of these data, we hoped to identify some of the gene changes associated with the major innovations in Viridiplantae evolution, such as multicellularity, transitions from marine to freshwater or terrestrial environments, maternal retention of zygotes and embryos, complex life history involving haploid and diploid phases, vascular systems, seeds and flowers.
Our RNA extraction protocols  and our RNA-Seq transcriptome assembly algorithms  have already been published. Here, we are publishing the second of two linked papers. The first is a review of the state-of-knowledge for Viridiplantae species relationships and our initial foray into the phylogenomics on a subset of 1KP . The other is a description of the websites that we created in order to provide access to the data (from raw reads to computed results), visualize the results, and perform custom analyses in conjunction with external data that the users can upload. An initial gene annotation is also provided, which focuses on the functional relationships between proteins and their associated metabolites.
Access to raw and processed data
Our initial phylogenomics effort used sequences from multiple sources. They include transcriptomes from 1KP representing 85 species, transcriptomes from other sources representing 7 species, and genomes representing an additional 11 species. A summary of these data sources is given in Table 1. We submitted all of the unassembled reads from the 1KP transcriptomes to the Short Reads Archive (SRA) under project accession PRJEB4921 “1000 Plant (1KP) Transcriptome: The Pilot Study.” Note that, with the exception of Eschscholzia californica, we sequenced only one sample per species.
To make it easier for others to reproduce our phylogenomics analyses, we are releasing our intermediate computations, not just the final results. Everything is hosted at the iPlant Data Store, a high performance, large capacity, distributed storage system. The contents include transcriptome assemblies, putative coding sequences, orthogroups (i.e., from the 11 reference genomes), as well as gene and species trees with related sequence alignments. There are quite a lot of files and their total sizes are not negligible; so before users begin to download these files, we suggest that they consult Table 2 for a description of what to expect.
At the simplest level, anonymous downloads are permitted from a designated area of the iPlant Data Store . However, much greater functionality is available through the iPlant resources that we describe in the following sections.
Visualization and custom analyses
To take full advantage of the iPlant computational infrastructure, it is necessary to first register at . Accounts are free, and in addition to 1KP data, users will find high performance computing and cloud-based services. Multiple access modalities are supported: anonymous and secure web interfaces, desktop clients and high-speed command lines. However, we feel that for most users the best option is the iPlant discovery environment (DE), a web-based interface that provides users with high-performance computing resources and data storage. Most contemporary web browsers are supported, including Safari v. 6.1, Firefox v. 24, and Chrome v. 34. The caveat is that some of these functionalities (see below) require Java 1.6.
To guide users through its resources, iPlant is constantly producing new tutorials and teaching materials, including live and recorded webinars. The full catalog can be found at . Here, we describe the new resources specifically created for 1KP.
Discovery environment (DE)
From the data window it is possible to download individual files or perform bulk downloads of multiple files and directories through a Java plugin. Note that for security reasons, some operating systems will not allow users to run Java applets. In this instance, a window will pop up to tell the user that there is a problem, and the user should follow the instructions that are given to configure an iDrop desktop  Figure 2.
It is possible to perform analyses directly in the DE using any of the 1KP files as input; for example, users can re-compute the sequence alignments and gene trees using different algorithms and parameters  Figure 3. More generally, users can select from a variety of applications in the Apps catalogue, which is constantly growing, and includes many popular bioinformatics tools for large-scale phylogenetics, genome-wide associations and next generation sequence analyses.
Species and gene trees can be explored with the iPlant tree viewer, Phylozoom, a newly developed web-based phylogenetic tree viewer that supports trees with hundreds of thousand leaves and allows for semantic zooming Figure 4. To access the tree viewer, users need only click on a tree file. This will open a preview window with two tabs: one for the tree’s newick string (a format for graph-theoretical trees as defined at ) and another for the web link that opens a window to the tree display. Notice that pop-ups must be enabled on the user’s browser.
To zoom in and expand the collapsed clades, click on the node of interest. To zoom out, click and drag the tree figure to the left. To zoom out completely, click the space bar. The web address is a unique identifier that can be shared with others to let them to visualize the tree.
For more advanced users wanting to perform more complicated procedures, iPlant capabilities are available from a command line. It is based on the integrated rule-oriented data system (iRODS) . All the user has to do is install a command line utility, icommands, which mimics UNIX and enables high-speed parallel data transfers. Instructions are available at .
Interactions and pathways
In addition to the tree-based species and gene relationships at the iPlant site, functional relationships between proteins and their associated metabolites are available from the Computational Biology Group at the University of Washington, developers of CANDO . Sequence similarity-based methods are used to map 1KP proteins to curated repositories of protein-protein interactions (i.e., BioGRID ) and biochemical pathways (i.e., Kyoto Encylopedia of Genes and Genomes [KEGG] ). The user can select any metabolic pathway defined by KEGG and, within this context, see all the 1KP proteins from their chosen species, with functional annotations inferred from KEGG. This website is at  Figure 5.
Note that, over the course of this project, there have been many improvements in the transcriptome assemblies. The phylogenomics work (now being published) was done with the SOAPdenovo algorithm. A second assembly was subsequently done with the newer SOAPdenovo-trans algorithm, which we incorporated into the newer interactions and pathways work. However, both sets of assemblies are available through the iPlant data store.
The rest of the 1KP data will be released, on much the same platform, along with our analyses of all one thousand species. Our scientific objectives are given at . We have always been open about our intentions, because we wanted to avoid conflict among the scientists who were already working with 1KP and offer early pre-publication access to other non-competing scientists. As soon as we see a draft of a paper, we track its progress through the review process at . Some of these papers have already been published, and more than a few required years of follow-up experiments, resulting for example in fundamental discoveries for molecular evolution  and (surprisingly) new tools for mammalian neurosciences .
Many of these studies were not anticipated when 1KP was conceived. We only knew that, just as there was value in sequencing every gene in a genome, despite not knowing a priori what many of the genes might do, there would be value in sequencing across an ancient and ecologically dominant clade, even when many of the species have no obvious economic or scientific value that would justify a genome sequencing effort. Transcriptomes were a less expensive way to explore plant diversity, and demonstrate value beyond the obvious species.
1,000 Plants project
Kyoto Encyclopedia of Genes and Genomes
National Science Foundation
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The 1000 Plants (1KP) initiative, led by GKW, is funded by the Alberta Ministry of Innovation and Advanced Education, Alberta Innovates Technology Futures (AITF), Innovates Centre of Research Excellence (iCORE), Musea Ventures, BGI-Shenzhen and China National GeneBank (CNGB). We thank the many people responsible for sample collection on 1KP and the staff at BGI-Shenzhen for doing our sequencing. Phylogenomic analyses were supported by the US National Science Foundation through the iPlant collaborative. CANDO was funded by an NIH Director’s Pioneer Award 1DP1OD006779-01.
The authors declare that they have no competing interests.
CWD, BRR, NWM, SWG, S Ma, BS, MM, DES, PSS, CR, LP, JAS, LD, DWS, JCV, TC, TMK, MR, RSB, MKD, and JLM collected the plant samples. NM, NJW, S Mi, NN, TW, SA, MB, JGB, MAG, EW, JPD, CWD, BR, HP, BRR, and JLM performed the phylogenomic analyses. NM, LHH, ZY, and EJC setup and maintained web-resources used to communicate data. LHH and RS performed the protein and KEGG pathway analyses. EJC, ZT, XW, XS, YZ, JW, and GKW generated the sequence data. GKW and JLM designed and oversaw the research. All authors read and approved the final manuscript.
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Matasci, N., Hung, L., Yan, Z. et al. Data access for the 1,000 Plants (1KP) project. GigaSci 3, 17 (2014) doi:10.1186/2047-217X-3-17