Fig. 1
From: INC-Seq: accurate single molecule reads using nanopore sequencing
Overview of the INC-Seq workflow. a Template molecules are circularized in optimized conditions, and the remaining linear molecules are removed. The circular products are amplified with RCA and sequenced on the MinION platform. b A subsequence from the raw nanopore reads is used as an anchor to scan the entire read for the location of repeating units. The repeating units flanked by adjacent anchor starting points are aligned, and a consensus sequence is constructed. c In INC-Seq library preparation, chimeras are expected through intermolecular ligation and template switching. Chimeras from template switching are likely to be detected by the anchor mapping protocol. Chimeras from intermolecular ligation were observed to be rare under the experimental conditions used in INC-Seq