Table 2 Transcriptome assembly and annotation statistics compared with previous P. astreoides transcriptome
From: Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages
Assembly components | P. astreoides + Symbiodinium sp. 2016 | P. astreoides alone 2016 | P. astreoides alone 2013 [3] | Symbiodinium sp. alone 2016 |
|---|---|---|---|---|
Genes | 717 454 | 95 294 | 29 422 | 145 570 |
Transcripts | 867 255 | 129 718 | 30 740 | 186 177 |
GC % | 44 | 40 | 42 | 48 |
Contig N10 | 3876 | 4642 | 1574 | 5230 |
Contig N20 | 2568 | 3321 | 1207 | 3701 |
Contig N30 | 1871 | 2487 | 949 | 2825 |
Contig N40 | 1386 | 1867 | 767 | 2236 |
Contig N50 | 1005 | 1358 | 661 | 1804 |
n under 200 | 0 | 0 | 2164 | 0 |
n over 1 k | 145 836 | 27 334 | 3274 | 67 354 |
n over 10 k | 319 | 58 | 0 | 260 |
Median contig length (bp) | 389 | 429 | 418 | 659 |
Average contig length (bp) | 685 | 811 | 550 | 1105 |
Maximum length (bp) | 28 297 | 19 877 | 8171 | 28 297 |
Minimum length (bp) | 201 | 201 | 100 | 201 |
Bases Ns | 0 | 0 | 924 | 0 |
Total assembled bases | 594 399 145 | 105 218 865 | 16 907 062 | 205 741 294 |
N50 (longest transcript per unigene) | 763 | 967 | 640 | 1623 |
Total assembled bases (longest transcript per unigene) | 430 507 117 | 65 529 888 | 15 807 055 | 145 440 520 |
No. of possible ORF | 578 372 | 49 348 | 18 351 | 221 299 |
No. of transcripts with ORF | 327 829 | 33 331 | 15 183 | 89 425 |
No. of all possible complete ORF | 223 741 | 26 802 | 3666 | 124 810 |
No. of transcripts with complete ORF | 123 339 | 17 277 | 2932 | 59 126 |
No. of transcripts with Swiss-Prot blast hit | 204 071 | 25 384 | 15 492 | 52 308 |