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Table 1 Summary of sample preparation

From: Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages

Source

N

Library

Sequencer

Cycles

# Reads

Swimming larvae

20 (2012), 12 (2014)

Illumina TruSeq mRNA Version 2 Library preparation Kit (2012); Illumina TruSeq Stranded mRNA Library preparation Kit LT (2014)

Illumina HiSeq 2000 flow cell using TruSeq SBS Version 3 reagents (2012); Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents (2014)

2 × 100 bp paired-end (2012); 2 × 150 bp paired-end (2014)

133 268 440 (2012) and 25 183 766 (2014)

Newly settled larvae

15 (2012), 10 (2014)

Illumina TruSeq mRNA Version 2 Library preparation Kit (2012); Illumina TruSeq Stranded mRNA Library preparation Kit LT (2014)

Illumina HiSeq 2000 flow cell using TruSeq SBS Version 3 reagents (2012); Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents (2014)

2 × 100 bp paired-end (2012); 2 × 150 bp paired-end (2014)

109 623 674 (2012) and 20 628 911 (2014)

Adult

1

Illumina TruSeq Stranded mRNA Library preparation Kit LT (2013)

Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents

2 × 100 bp paired-end

104 806 800