Skip to main content

Table 1 Summary of sample preparation

From: Transcriptome of the Caribbean stony coral Porites astreoides from three developmental stages

Source N Library Sequencer Cycles # Reads
Swimming larvae 20 (2012), 12 (2014) Illumina TruSeq mRNA Version 2 Library preparation Kit (2012); Illumina TruSeq Stranded mRNA Library preparation Kit LT (2014) Illumina HiSeq 2000 flow cell using TruSeq SBS Version 3 reagents (2012); Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents (2014) 2 × 100 bp paired-end (2012); 2 × 150 bp paired-end (2014) 133 268 440 (2012) and 25 183 766 (2014)
Newly settled larvae 15 (2012), 10 (2014) Illumina TruSeq mRNA Version 2 Library preparation Kit (2012); Illumina TruSeq Stranded mRNA Library preparation Kit LT (2014) Illumina HiSeq 2000 flow cell using TruSeq SBS Version 3 reagents (2012); Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents (2014) 2 × 100 bp paired-end (2012); 2 × 150 bp paired-end (2014) 109 623 674 (2012) and 20 628 911 (2014)
Adult 1 Illumina TruSeq Stranded mRNA Library preparation Kit LT (2013) Illumina HiSeq 2500 Rapid Run flow cell (v1) with Rapid SBS reagents 2 × 100 bp paired-end 104 806 800