Sequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome

GigaScience

Table 1 Comparison of assembled lepidopteran transcriptomes

	Chilo suppressalis	Heliothis virescens	Plutella xylostella	Pectinophora gossypiella
Platform	Illumina	Illumina, Roche, 454, Sanger	Illumina	Illumina
Assembled reads	39,400,002	212,987,028	39,764,230	18,623,508
Average read size (bp)	90	Variable	90	72
Number of contigs	37,040	63,648	213,674	46,458
Contigs per 1000 reads	0.94	0.30	5.4	2.5
Mean contig size (bp), range	497, 201–9744	383, 80–2000	189, nr	770, 224–14,619
Sequences with e-value <10⁻⁵	15,446	29,978	28,768	21,715
Number of e-value <10⁻⁵ hits Per 1000 reads	0.39	0.14	0.72	1.2
GC (%)	42	nr^a	nr	39
N₅₀ transcript length (bp)	nr	1031	262	1153
Pipeline	Trinity	SeqMan Ngen v2.1	Trinity	Trinity
Reference	[14]	[15]	[13]	This study

ISSN: 2047-217X