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Table 1 Schistocephalus solidus specimens from three different developmental stages used to generate the de novo reference transcriptomeb

From: Transcriptome sequences spanning key developmental states as a resource for the study of the cestode Schistocephalus solidus, a threespine stickleback parasite

Sample ID Mass (mg) Stagea Library size (no. raw reads) Platform Ave. read quality (PHRED score)
ssol.cltw.NI.05 1.7 NI 95.7 M HiSeq 2000 37
ssol.cltw. NI.08 1.5 NI 57.6 M HiSeq 2000 37
ssol.cltw. NI.12 5.2 NI 29.5 M HiSeq 2000 37
ssol.cltw. NI.13 1.8 NI 69.6 M HiSeq 2000 37
ssol.cltw. NI.14 3.4 NI 59.8 M HiSeq 2000 37
ssol.cltw. NI.22 7.7 NI 49.1 M HiSeq 2000 37
ssol.cltw. NI.26 13.1 NI 53.7 M HiSeq 2000 37
ssol.cltw. NI.63 102 I 37.5 M HiSeq 2000 37
ssol.cltw. NI.67 90.1 I 42.3 M HiSeq 2000 37
ssol.cltw. I.98–1 101.2 I 36.8 M HiSeq 2000 37
ssol.cltw.I.98–2 108.1 I 56.3 M HiSeq 2000 37
ssol.cltw.A.01 164.5 A 38.8 M MiSeq 38
ssol.cltw.A.03 321 A 61.2 M HiSeq 2000 37
ssol.cltw.A.07 329.4 A 50.2 M HiSeq 2000 37
ssol.cltw.A.12 356 A 51.7 M HiSeq 2000 37
  1. a NI non-infective, I infective, A adult
  2. bWorms in bold were selected as the three representative samples to be used to perform the initial raw de novo assembly. All 14 HiSeq libraries were then used to eliminate redundancy in the final dataset and increase assembly quality, while sample cltw.a-01 (in italic) was used to perform preliminary optimization tests on assembly parameters