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Table 2 Taxonomy assignment of 2d reads derived from 16S rDNA sequencing using MinION™

From: Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

Genus assignment At 80 % identity (SINA) At 70 % identity (SINA) At 60 % identity (SINA) Species assignment (Blasta)
Bacillus 3 3 4 B. cereus
Escherichia 61 93 103 E. coli , E. fergusonii, Shigella flexneri
Streptococcus 69 96 111 S. mutans , S. gordonii, S. agalactiae
Lactobacillus 2 2 3 L. gasseri , L. fornicalis
Staphylococcus 37 55 60 S. epidermidis , S. capitis, S. aureus
Rhodobacter 4 8 10 R. sphaeroides
Clostridium 0 2 2 N/A
Listeria 0 0 1 N/A
Cronobacter* 0 1 1 N/A
Yersinia* 0 0 1 N/A
Citrobacter* 0 0 1 C. koseri*, C. muytjensii*
Alteromonas* 0 0 1 N/A
Burkholderia* 0 0 1 N/A
Sulfitobacter* 0 0 1 N/A
Total assigned 176/601 260/601 300/601 172/601
  1. aBlast searching against the NCBI bacterial 16S database. Valid assignments were based on E-values < 1e−03 and alignments larger than 800 bp. N/A means no species was found for the respective genus. Species matching with those present in the mock community are underlined and in bold. Rows with genera, species, and read counts highlighted with asterisk (*) indicate they are not expected to be present in the mock community
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