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Table 1 Statistics of the mapping process using 16S rDNA reads produced by MinION™

From: Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

Organism

Mapped reads

Mapped bases

Strand mappinga

Max lengthb

Mean length

Variants after assembly

Consensus

rRNAgenec

Assembled 16Sd

Acinetobacter baumannii

98

99,352

0.46:0.54

1390

1013

32

1415

1529

0.93

Actinomyces odontolyticus

79

73,480

0.49:0.51

1377

930

8

1407

1528

0.92

Bacillus cereus

144

151,668

0.46:0.54

1419

1053

31

1415

1508

0.94

Bacteroides vulgatus

33

29,499

0.58:0.42

1346

893

25

1403

1510

0.93

Clostridium beijerinckii

97

99,476

0.46:0.54

1393

1025

13

1408

1505

0.94

Deinococcus radiodurans

73

69,940

0.45:0.55

1390

958

8

1398

1502

0.93

Enterococcus faecalis

149

153,581

0.50:0.50

1398

1030

8

1444

1549

0.93

Escherichia coli

167

181,084

0.45:0.55

1398

1084

0

1434

1542

0.93

Helicobacter pylori

67

62,838

0.46:0.54

1390

937

11

1411

1498

0.94

Lactobacillus gasseri

123

128,120

0.51:0.49

1407

1041

0

1467

1579

0.93

Listeria monocytogenes

139

140,478

0.50:0.50

1343

1010

13

1374

1486

0.92

Neisseria meningitidis

87

86,916

0.48:0.52

1390

999

11

1433

1544

0.93

Propionibacterium acnes

75

70,160

0.48:0.52

1375

935

21

1401

1525

0.92

Pseudomonas aeruginosa

113

120,520

0.55:0.45

1398

1066

14

1425

1536

0.93

Rhodobacter sphaeroides

95

89,750

0.52:0.48

1416

944

5

1352

1463

0.92

Staphylococcus epidermidis

164

177,084

0.51:0.49

1423

1079

0

1443

1540

0.94

Staphylococcus aureus

163

179,477

0.51:0.49

1423

1101

1

1435

1554

0.92

Streptococcus agalactiae

156

166,420

0.52:0.48

1411

1066

5

1439

1551

0.93

Streptococcus mutans

196

221,682

0.47:0.53

1411

1131

2

1440

1552

0.93

Streptococcus pneumoniae

154

168,657

0.52:0.48

1411

1095

2

1442

1560

0.92

  1. aProportion of reads mapped against the forward and complementary strand, respectively
  2. bMaximum length of reads mapped
  3. cLength of the 16S sequence used as reference
  4. dNumbers are generated from Consensus/rRNA gene ratio