Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

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Table 1 Statistics of the mapping process using 16S rDNA reads produced by MinION™

Organism	Mapped reads	Mapped bases	Strand mapping^a	Max length^b	Mean length	Variants after assembly	Consensus	rRNAgene^c	Assembled 16S^d
Acinetobacter baumannii	98	99,352	0.46:0.54	1390	1013	32	1415	1529	0.93
Actinomyces odontolyticus	79	73,480	0.49:0.51	1377	930	8	1407	1528	0.92
Bacillus cereus	144	151,668	0.46:0.54	1419	1053	31	1415	1508	0.94
Bacteroides vulgatus	33	29,499	0.58:0.42	1346	893	25	1403	1510	0.93
Clostridium beijerinckii	97	99,476	0.46:0.54	1393	1025	13	1408	1505	0.94
Deinococcus radiodurans	73	69,940	0.45:0.55	1390	958	8	1398	1502	0.93
Enterococcus faecalis	149	153,581	0.50:0.50	1398	1030	8	1444	1549	0.93
Escherichia coli	167	181,084	0.45:0.55	1398	1084	0	1434	1542	0.93
Helicobacter pylori	67	62,838	0.46:0.54	1390	937	11	1411	1498	0.94
Lactobacillus gasseri	123	128,120	0.51:0.49	1407	1041	0	1467	1579	0.93
Listeria monocytogenes	139	140,478	0.50:0.50	1343	1010	13	1374	1486	0.92
Neisseria meningitidis	87	86,916	0.48:0.52	1390	999	11	1433	1544	0.93
Propionibacterium acnes	75	70,160	0.48:0.52	1375	935	21	1401	1525	0.92
Pseudomonas aeruginosa	113	120,520	0.55:0.45	1398	1066	14	1425	1536	0.93
Rhodobacter sphaeroides	95	89,750	0.52:0.48	1416	944	5	1352	1463	0.92
Staphylococcus epidermidis	164	177,084	0.51:0.49	1423	1079	0	1443	1540	0.94
Staphylococcus aureus	163	179,477	0.51:0.49	1423	1101	1	1435	1554	0.92
Streptococcus agalactiae	156	166,420	0.52:0.48	1411	1066	5	1439	1551	0.93
Streptococcus mutans	196	221,682	0.47:0.53	1411	1131	2	1440	1552	0.93
Streptococcus pneumoniae	154	168,657	0.52:0.48	1411	1095	2	1442	1560	0.92

^aProportion of reads mapped against the forward and complementary strand, respectively
^bMaximum length of reads mapped
^cLength of the 16S sequence used as reference
^dNumbers are generated from Consensus/rRNA gene ratio

ISSN: 2047-217X