Table 2 Transcriptome assembly and annotation statistics compared with previous Lygus transcriptomes
Transcriptome | ||||
|---|---|---|---|---|
L. lineolaris a | L. hesperus (454)b | L. hesperus (thermal)c | L. hesperus (current)d | |
Assembly | ||||
Total no. read pairs | 262,555 | 1,429,818 | 144,898,116 | 437,850,562 |
Normalized reads (in silico normalization) | - | - | 16,191,383 | 32,342,216 |
Total no. transcripts | 6970 | 36,131 | 45,706 | 22,022 |
Average transcript length | 392 (100–3466) | 725 (2–13,480) | 2237 (300–23,322) | 2073 (297–23,350) |
Total assembled bases (all transcripts) | - | 32,252,977 | 102,246,199 | 45,687,929 |
Total assembled bases (longest transcript per unigene) | - | 28.8 Mb | 39.8 Mb | 31.6 Mb |
N50 (all transcripts) | - | 2430 | 2989 | 2610 |
N50 (longest transcript per unigene) | - | 1849 | 2638 | 2726 |
%GC | - | 0.41 | 0.44 | 0.45 |
Proteins with complete ORF (%) | - | - | - | 13,689 (62.1 %) |
Annotation | ||||
No. transcripts with a BLAST hit | 3126 (44.9 %) | 19,393 (54 %) | - | 16,942 (76.9 %) |
No. transcripts with GO term | 2196 (31.5 %) | 7898 (21 %) | - | 12,114 (54.9 %) |
PFAM | - | 3705 (22.2 %) | - | 14,575 (66.1 %) |