Skip to main content

Table 2 Transcriptome assembly and annotation statistics compared with previous Lygus transcriptomes

From: De novo construction of an expanded transcriptome assembly for the western tarnished plant bug, Lygus hesperus

   Transcriptome  
L. lineolaris a L. hesperus (454)b L. hesperus (thermal)c L. hesperus (current)d
Assembly     
 Total no. read pairs 262,555 1,429,818 144,898,116 437,850,562
 Normalized reads (in silico normalization) - - 16,191,383 32,342,216
 Total no. transcripts 6970 36,131 45,706 22,022
 Average transcript length 392 (100–3466) 725 (2–13,480) 2237 (300–23,322) 2073 (297–23,350)
 Total assembled bases (all transcripts) - 32,252,977 102,246,199 45,687,929
 Total assembled bases (longest transcript per unigene) - 28.8 Mb 39.8 Mb 31.6 Mb
 N50 (all transcripts) - 2430 2989 2610
 N50 (longest transcript per unigene) - 1849 2638 2726
 %GC - 0.41 0.44 0.45
 Proteins with complete ORF (%) - - - 13,689 (62.1 %)
Annotation     
 No. transcripts with a BLAST hit 3126 (44.9 %) 19,393 (54 %) - 16,942 (76.9 %)
 No. transcripts with GO term 2196 (31.5 %) 7898 (21 %) - 12,114 (54.9 %)
 PFAM - 3705 (22.2 %) - 14,575 (66.1 %)
  1. Data from: aMagalhaes et al. 2013 [11]; bHull et al. 2013 [2]; cHull et al. 2014 [3]