Fig. 3

FAM84B knockdown dramatically inhibited cell proliferation, cell migration and invasion. a The knockdown efficiency of FAM84B was verified by western blotting analysis. Actin was used as a loading control. b MTT growth assay shows an increase in proliferation following knockdown of FAM84B in KYSE150 and TE-1 cells. Data represent the mean ± SD; At least three independent experiments were performed; each experiment was performed in triplicate. Statistical analysis was done using a two-sided t-test. *p < 0.05, **p < 0.01. c,d In vitro migration/invasion assay with three experimental replicates revealed that knockdown of FAM84B significantly promotes cell migration and invasion in KYSE150 and TE-1 cells. SCR indicates non-specific control siRNA. Each experiment was performed in triplicate; data are mean ± SD. *p < 0.05; **p < 0.01