Fig. 2

FAM84B is amplified and overexpressed in ESCC tumors. a Focally amplified (red) region containing FAM84B of the WGS set viewed by IGV is plotted along the chromosome. b Representative immunofluorescence images show signals produced from FISH analyses using probes specific to chromosome 2 (red) and FAM84B (green) in normal, preclinical atypical hyperplasia tissue and an ESCC sample. Scale bars, 5 μm. The bar graph (right panel) shows the percentage of indicated cases with more than two FAM84B signals in each group. c FAM84B is highly expressed in ESCC tumors. Representative immunohistochemistry images show FAM84B expression in ESCC tumors and matched normal tissues. The right image shows FAM84B expression in one slide with tumor cells and adjacent normal cells. The bar graph (right panel) shows the percentage of indicated cases with varying FAM84B expression levels in the 104 patient cohort. FAM84B expression level was based on subjective assessment of immunohistochemical staining intensity (see Online Methods). Scale bars, 400 μm. ***p < 0.001. d Left panel: Representative images displaying cytoplasmic positivity of FAM84B in normal esophagus tissue, atypical hyperplasia tissue, ESCC in situ and invasive ESCC tissues from large-scale tissue microarraays (OD-CT-DgEso01-001, Shanghai Outdo Biotech Detail information of cases was shown in Additional file 16: Table S9). Right panel: Cytoplasm expression significantly increases in atypical hyperplasia tissues, ESCC in situ and invasive ESCC tissues compared with that of normal esophagus tissues based on a judgment of immunohistochemistry staining intensity (χ²-test). The normal esophagus tissues include 10 from the OD-CT-DgEso01-001 array and 104 matched normal esophagus tissues from our sequenced cohort