Single-molecule optical genome mapping of a human HapMap and a colorectal cancer cell line

GigaScience

Table 2 Summary of MapCard statistics of GM12878

MapCard ID	F^a	Input maps^b (theoretical genome coverage)	Average Argus quality score	Average DNA molecule size (kb)	Average # of fragments	Average fragment size (kb)	OPTIMA alignment rate	Yield (genome coverage)^c	Average digestion rate^c	Average false/ extra cut rate^c	Ratio small missing fragments (≤2 kb)^c
21157LB	(r)	73365 (7.2×)	0.50	295	18	16.5	0.253	2.0×	0.659	0.736	0.139
	(s)	38483 (4.7×)	0.53	368	22	17.0	0.357	1.7×	0.650	0.733	0.133
21159LB	(r)	75761 (7.6×)	0.47	300	17	17.4	0.190	1.6×	0.628	0.723	0.129
	(s)	41236 (5.1×)	0.50	370	21	17.8	0.268	1.3×	0.618	0.718	0.124
21431LB	(r)	93896 (8.6×)	0.52	274	17	15.8	0.200	1.9×	0.676	0.773	0.187
	(s)	43667 (5.1×)	0.54	348	21	16.3	0.303	1.5×	0.665	0.768	0.184
21443LB	(r)	66857 (6×)	0.51	271	17	15.8	0.192	1.3×	0.674	0.771	0.175
	(s)	29991 (3.5×)	0.53	346	21	16.3	0.292	1.0×	0.661	0.772	0.168
Total	(r)	309879 (29.4×)	0.50	285	17	16.4	0.209	6.8×	0.660	0.751	0.158
	(s)	153377 (18.3×)	0.52	359	21	16.9	0.310	5.5×	0.649	0.747	0.152

^ar: inclusion of DNA molecules with ≥10 fragments and ≥150 kb in length; s: inclusion of DNA molecules with ≥12 fragments and ≥250 kb in length
^bfragmented DNA molecules
^cof OPTIMA aligned data

ISSN: 2047-217X