Fig. 6

Sizing. a The two pixel wide mask used to compute the integrated fluorescence values for fragments. The darker grey pixels represent the skeletal pixels. Shown here is a fragment that spans 7 pixels. b An example three fragment, Bsu36I digested, Lambda DNA standard (≈48.5 kb) used to estimate C _kb . The expected fragment sizes (in kilobases) are: 26.718, 7.601 and 14.183. Standards can be selected based on the experiment. c A portion of a Rmap with fragment sizes using the estimated C _kb to determine sizes in kilobases from integrated fluorescence values