Fig. 2

A high sensitivity, accuracy and reproducibility of MIRALCS. a Comparison of gene number between single cell (the smaller circle) and the 5 ng bulk sample (the larger circle). The left, a typical cell; the right, 5 randomly selected cells (randomly sampling 0.4 million reads per cell) vs. the 5 ng bulk sample (2 million reads). b Gene detection in MIRALCS single-cells, regular tube-based single cells and 5 ng bulk RNA sample. c The distribution of gene number on gene expression along sequencing depths. d The correlation of the mean expression (FPKM) and the number of input molecules of spike-ins of all MIRALCS single-cell libraries. e The reads coverage along the transcript position from 5′ to 3′end. Error bar stands for the standard deviation. f The correlation of spike-ins expression (FPKM) between two randomly selected MIRALCS single cells. g Heat map of correlation coefficients of spike-ins expression levels with input molecules >1 for each library (n = 19). h The correlation of gene expression (FPKM) between technical replicates. Left: two randomly selected MIRALCS 10 pg replicates. Right: two randomly selected tube-based 10 pg replicates. i The pair-wise correlation in MIRALCS 10 pg RNA replicates and tube-based 10 pg RNA replicates