Fig. 1

K-mer spectrum for the genome sequence of a male Pogona vitticeps (ZZ). Sequencing DNA derived from the short-insert libraries (250, 500, 800 bp) yielded 98.35 Gbases of clean data in the form of 125 bp reads, which generated 76.89x109 17-mer sequences. The solid line shows the k-mer spectrum (percentage frequency against k-mer copy number). The second mode (copy number 48.5) represents homozygous single copy sequence, whereas the first mode (24.5), half the copy number of the first, represents heterozygous single copy sequence. Heterozygosity is high, which complicated assembly