Figure 3

Cases of positively selected sites and non-neutral penguin-specific amino acid changes. (A) Positively selected sites in emperor penguin CNGB1 protein sequence. Cytoplasmic and transmembrane regions are separated by the dashed lines. Blue shading represents the membrane-spanning helix, and the cAMP binding domain is shown in grey. The posterior probabilities were calculated using BEB method in CODEML. (B) Positively selected sites in the FASN protein in the Adélie lineage (green dots) and the ancestral lineage (blue dots). The molecular binding domains of FASN are shown in light red, whereas the major catalytic domains are shown in grey. From left to right, beta-ketoacyl synthase (KS), acyl and malonyl transferases (MAT), enoyl reductase (ER), beta-ketoacyl reductase (KR), and thioesterase (TE). The posterior probabilities were calculated using BEB method in CODEML. (C) Non-neutral penguin-specific amino acid changes in the EVC2 protein. One substitution site is located in the Pfam domain EVC_like (PF12297).