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Table 1 Comparisons between different genome editing technologies

From: Advances in genome editing technology and its promising application in evolutionary and ecological studies

  Site-specific recombinase technology ZFN TALEN CRISPR/Cas9
Efficiency 0.03%-0.3% 0 ~ 12% 0 ~ 76% 0 ~ 81%
Off-target effect Less observed off More potential off-target effects than TALENs Less observed off-target effects More potential off-target effects than TALENs and ZFNs
Applicable organism Only in metazoan metazoan、plants metazoan、plants metazoan、plants
Possible target sites Needs specific sites(loxP, FLP et al.) 500 bp 36 bp 8 bp
Methylation sensitive Not sure Sensitive Sensitive Not sensitive
Multiplexable No Rarely used Rarely used Yes
Programmable Limited Moderate Moderate/high Moderate/high
  1. Note: We compare the advantages between site-specific recombinase technology, ZFN, TALEN and CRISPR/Cas9 in several aspects. ZFN, TALEN and CRISPR/Cas9 have much higher efficiencies and effects in various organisms.
  2. ZFN, Zinc Finger Nuclease; TALEN, Transcription Activator-Like Effector Nuclease; CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats.