Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification

Table 1 Sample composition, sequencing information and COI recovery rates of the bulk insect sample

Number of Individuals	73
Number of COI barcodes obtained	69
Number of MOTUs (2%)	37
Raw data size (Gb)	15.5
High quality data size (Gb)	13.2
Discovery rate (with reference)	97%
Discovery rate (no reference)	92%
Assembly coverage rate (% MT genomes)	74%
Total length and percentage of COI genes ¹	51,768 (96%)
Number of assembled mitochondrial genes ²	613

¹ The total length (bp) of assembled COI genes and the percentage of assembled COI sequences for 37 full-length COI genes (~ 1,530 bp for each species).
² Note that a small portion of the genes were assembled into two scaffolds.

ISSN: 2047-217X