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Table 1 Tissue standards ( italicized ) for vertebrate genomics corresponding to storage and quality of target materials (bolded)

From: Tissue sampling methods and standards for vertebrate genomics

 

Four-star

Three-star

Two-star

One-star

DNA quantity

    

1 mg

X

X

  

> 700 μg

  

X

 

≤ 700 μg a

   

X

Target materials

    

Cell lines/tissue culture

X

   

RNA

X

X

  

DNA

X

X

X

X b

Specimen type c

    

Live/freshly euthanized

X d

X

  

Salvaged

  

X

X

Voucher

  

X

X

Storage

    

RNAlater®

X

   

DNAgard/DNAstable®

X

   

≤ −130°C

X

   

≥ −80°C

 

X

  

≥ −20°C

  

X

 

Ethanol

   

X

  1. aSmaller quantities (30 to 100 μg) from multiple individuals of the same species will support light-coverage sequencing for single-nucleotide polymorphism discovery.
  2. bHigh-quality or slightly degraded DNA of small quantities will not likely be sufficient for whole-genome sequencing; these samples may supplement whole-genome sequencing efforts of higher-quality samples.
  3. cStandards will vary depending on tissue selection and natural history of the specimen.
  4. dFour-star samples should also include reference species for aligning de novo sequences of closely related species (see text for more details).
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