|
Four-star
|
Three-star
|
Two-star
|
One-star
|
---|
DNA quantity
| | | | |
1 mg
|
X
|
X
| | |
> 700 μg
| | |
X
| |
≤ 700 μg a
| | | |
X
|
Target materials
| | | | |
Cell lines/tissue culture
|
X
| | | |
RNA
|
X
|
X
| | |
DNA
|
X
|
X
|
X
|
X b
|
Specimen type
c
| | | | |
Live/freshly euthanized
|
X d
|
X
| | |
Salvaged
| | |
X
|
X
|
Voucher
| | |
X
|
X
|
Storage
| | | | |
RNAlater®
|
X
| | | |
DNAgard/DNAstable®
|
X
| | | |
≤ −130°C
|
X
| | | |
≥ −80°C
| |
X
| | |
≥ −20°C
| | |
X
| |
Ethanol
| | | |
X
|
-
aSmaller quantities (30 to 100 μg) from multiple individuals of the same species will support light-coverage sequencing for single-nucleotide polymorphism discovery.
-
bHigh-quality or slightly degraded DNA of small quantities will not likely be sufficient for whole-genome sequencing; these samples may supplement whole-genome sequencing efforts of higher-quality samples.
-
cStandards will vary depending on tissue selection and natural history of the specimen.
-
dFour-star samples should also include reference species for aligning de novo sequences of closely related species (see text for more details).