Table 1 Relative cost of regenerating sequences for different classes of experiments
Class | Description | Example for DNA sequencing | Example for Imaging |
|---|---|---|---|
1 | Historical sampling of environment or time point-specific elements | Environmental genomics studies with a longitudinal component; Pathogen sequencing from epidemics | Earth imaging; environmental imaging for longitudinal studies |
2 | Very rare objects | Ancient DNA specimens; forensic samples | Fossils; rare meteorites |
3 | Longitudinal studies which could in theory be rerun in the future but have a > 10 year horizon to recreate | RNA-seq and DNA-seq from a prospective cohort; environmental sequencing of a specific field trial/intervention in an environment | MRI scans from a prospective cohort; cell imaging from a cohort |
4 | Samples acquired from patients or animals with a high individual acquisition cost, but a conceptually continuous generation | Cancer DNA sequencing | Histology samples from Cancer |
5 | A complex experiment with > 6 month resource development | RNA-seq on a specifically created mouse gene knockout (mouse colonies stored) | Cell imaging on a specific RNAi library |
6 | A routine experiment with < 6 month resource development | RNA-seq of a standard cell line | Routine imaging of Drosophila embryos |
7 | Verification experiment as a component in an overall flow | Resequencing of insert vector | Imaging of cell lines to determine confluence levels |