Circadian rhythms have significant effects on leaf-to-canopy scale gas exchange under field conditions
- Víctor Resco de Dios1Email author,
- Arthur Gessler2, 3,
- Juan Pedro Ferrio1, 4,
- Josu G. Alday1, 5,
- Michael Bahn6,
- Jorge del Castillo1,
- Sébastien Devidal7,
- Sonia García-Muñoz8,
- Zachary Kayler3, 9,
- Damien Landais7,
- Paula Martín-Gómez1,
- Alexandru Milcu7, 10,
- Clément Piel7,
- Karin Pirhofer-Walzl3, 11, 12,
- Olivier Ravel7,
- Serajis Salekin13, 14,
- David T. Tissue15,
- Mark G. Tjoelker15,
- Jordi Voltas1 and
- Jacques Roy7
© The Author(s). 2016
Received: 1 July 2016
Accepted: 20 September 2016
Published: 20 October 2016
Molecular clocks drive oscillations in leaf photosynthesis, stomatal conductance, and other cell and leaf-level processes over ~24 h under controlled laboratory conditions. The influence of such circadian regulation over whole-canopy fluxes remains uncertain; diurnal CO2 and H2O vapor flux dynamics in the field are currently interpreted as resulting almost exclusively from direct physiological responses to variations in light, temperature and other environmental factors. We tested whether circadian regulation would affect plant and canopy gas exchange at the Montpellier European Ecotron. Canopy and leaf-level fluxes were constantly monitored under field-like environmental conditions, and under constant environmental conditions (no variation in temperature, radiation, or other environmental cues).
We show direct experimental evidence at canopy scales of the circadian regulation of daytime gas exchange: 20–79 % of the daily variation range in CO2 and H2O fluxes occurred under circadian entrainment in canopies of an annual herb (bean) and of a perennial shrub (cotton). We also observed that considering circadian regulation improved performance by 8–17 % in commonly used stomatal conductance models.
Our results show that circadian controls affect diurnal CO2 and H2O flux patterns in entire canopies in field-like conditions, and its consideration significantly improves model performance. Circadian controls act as a ‘memory’ of the past conditions experienced by the plant, which synchronizes metabolism across entire plant canopies.
KeywordsCircadian clock Ecological memory Net ecosystem exchange Scaling Stomatal conductance models Photosynthesis Transpiration
Terrestrial ecosystems play a major role in global carbon and water cycles. Current estimates suggest that ~30 % of fossil fuel emissions are sequestered by land , and that ~60 % of annual precipitation is returned to the atmosphere through evapotranspiration; a flux largely dominated by transpiration . There is a long tradition within Earth Sciences research on deciphering the mechanisms underlying diurnal variations in photosynthesis and transpiration [3–6]. This research has mostly focused on direct physiological responses to the environment; i.e., understanding how photosynthetic machinery and stomatal function respond and react to changes in radiation, temperature, vapor pressure deficit, and other environmental drivers.
A smaller body of research has sought to disentangle whether, apart from direct responses to exogenous factors, endogenous processes could also play a role . It has been documented, for instance, that for a given level of water potential and abscisic acid (ABA) concentration, stomatal conductance is higher in the morning than it is in the afternoon . The circadian clock controls this phenomenon ; an endogenous timer of plant metabolism that controls the temporal pattern of transcription in photosynthesis, stomatal opening, and other physiological processes . Additional processes create endogenous flux variation, such as carbohydrate accumulation or apparent feed-forward stomatal responses [3, 10], but only the circadian clock shows a 24 h oscillation.
Ample evidence indicates the circadian regulation of leaf photosynthesis and conductance under controlled environmental conditions [11, 12]. However, processes regulating fluxes at the leaf scale will not necessarily also regulate fluxes at the canopy or ecosystem scales – not all processes relevant at one scale also act upon the ‘next’ scale . Unfortunately, research on the circadian regulation of photosynthesis and transpiration within field settings has received limited attention. Those studies mentioning circadian regulation often consider it to be a negligible driver at canopy or ecosystem scales [14, 15], although there are a few notable exceptions [16, 17].
Direct assessments of the circadian regulation of gas exchange over entire plant canopies are complicated because of logistical constraints (e.g., the need to control all sources of environmental variation, including radiation, over entire ecosystems). Instead, the scarce attempts to infer circadian regulation of carbon assimilation in the field at canopy or ecosystem scales have been achieved indirectly by filtering flux tower data, and have obtained circumstantial support for a significant effect of circadian regulation over net ecosystem CO2 exchange in the field [18, 19]. Others, working with nocturnal transpiration, have additionally documented that circadian regulation, over nocturnal stomatal conductance, affects the transpiration stream in whole trees  or even entire plant canopies .
Understanding whether or not circadian regulation in diurnal gas exchange is important at the leaf-level to the canopy-level scales requires an understanding of the potential implications of the circadian clock as a driver of ecosystem flux dynamics; there is some expectation that a dilution of circadian effects will occur as we move up in scale. Circadian rhythms are entrained by environmental cues of light and temperature; therefore, at the canopy scale, different leaves will experience different light and temperature cues. Hence, we could observe uncoupled circadian rhythms in different leaves within and across plants, potentially diluting any circadian effects at canopy scales.
Moreover, along with the capacity to detect circadian rhythms at canopy scales, additional studies are required that include circadian regulation in gas exchange modeling. To our knowledge, the only study that has so far tested the relevance of circadian rhythmicity for modeling gas exchange in the field concluded that, “circadian rhythms have insignificant effects on plant gas exchange under field conditions” . This was a pioneer study that, for the first time, took research on circadian rhythms outside of lab settings and worked with a non-model species from wetland and understory environments (Saururus cernuus L). The researchers  measured leaf-level fluxes under “constant environmental conditions” (i.e., when temperature, radiation, and other environmental drivers do not change over time) and documented a 24-h oscillation in gas exchange within growth chambers, consistent with circadian regulation of gas exchange. Subsequently, they tested whether or not circadian regulation would be significant in the field by adding a sinusoidal variation to a biochemical model of gas exchange, and then comparing modeled output with field-measured data. Under these field conditions, they observed that model goodness-of-fit increased by only 1 %; therefore it was concluded that circadian regulation of gas exchange in the field was insignificant. These negative results may partly explain the lack of interest in the circadian regulation of gas exchange in the field. However, this study was focused on photosynthesis and, to our knowledge, we are unaware of attempts to include circadian regulation in stomatal conductance models.
Here, we use large macrocosms within an experimental Ecotron  to monitor leaf and canopy gas exchange under field-like and constant environmental conditions in bean (Phaseolus vulgaris) and cotton (Gossypium hirsutum) canopies. We tested i) whether circadian regulation in photosynthesis and daytime stomatal conductance scales up from leaf-level to canopy-level; and ii) whether adding a circadian oscillator into well-known stomatal models would significantly increase model fit with observed data.
Diurnal variation in canopy and ecosystem fluxes is largely attributed to the direct environmental effects of photosynthetically active radiation (PAR), air temperature (Tair), and vapor pressure deficit (VPD) on physiological processes [3–6]. Currently lacking in the literature is an experiment acting as a ‘control’, whereby fluxes over entire plant canopies or ecosystems are measured under constant environmental conditions. Addressing this deficit, we assessed assimilation and transpiration in entire canopies under a fluctuating environment, and compared this with constant environmental conditions, to understand what diurnal range in canopy CO2 and H2O fluxes can be recreated, fully independently of environmental change. We hypothesized that if data revealed a temporal pattern under constant environmental conditions, also following a sinusoidal oscillation and with a ~24 h cycle, this would indicate circadian regulation at canopy scales. Additionally, we collected leaf-level gas exchange data to clarify whether considering circadian regulation would improve the performance of stomatal conductance models.
The experiment was performed at the Macrocosms platform of the Montpellier European Ecotron, Centre National de la Recherche Scientifique (CNRS, France). We used 12 outdoor macrocosms (six planted with bean and six with cotton) where the main abiotic drivers (air temperature, humidity, and CO2 concentration) were automatically controlled. Each macrocosm was designed as an open gas exchange system to continuously measure CO2 net ecosystem exchange by measuring air flow at the inlet of each dome (using a thermal mass flowmeter; Sensyflow iG, ABB, Zurich, Switzerland) and by sequentially (every 12 min) measuring the CO2 concentration at each inlet and outlet using a multiplexer system coupled with two LI-7000 CO2/H2O analyzers (LI-COR Biosciences, Lincoln, NE, USA). Substantial internal air-mixing within the dome (two volumes per min) reduced the canopy boundary layer resistance and minimized the CO2 concentration gradients within the dome. Belowground fluxes were prevented from mixing with canopy air by covering the soil with a plastic sheet during the entire experimental period. A slight atmospheric over-pressure (5–10 Pa) applied in the domes forced some air to flow below the plastic sheet (through the slits made for the plant stems). This air flow flushed away the CO2 respired by the soil and minimized potential mixing of soil respiration fluxes with aboveground fluxes. Indeed, we observed negligible CO2 flux at the onset of the experiment (immediately after seed germination, when there was no significant carbon assimilation), indicating no effect of soil CO2 efflux on the canopy above the plastic sheet. Transpiration was measured continuously by weighing lysimeters with four shear beam load cells per lysimeter (CMI-C3 Precia-Molen, Privas, France), and calculated from the slope of the temporal changes in mass using a generalized additive model with automated smoothness selection . Further details on Ecotron measurements are described elsewhere [23, 25].
For each crop, three macrocosms were dedicated to leaf-level measurements (researchers entered periodically) and the remaining three ‘undisturbed’ macrocosms were dedicated to continuous canopy gas exchange measurements. At the end of each experiment, leaf area was measured in five randomly selected plants from each macrocosm, using a leaf area meter (LI-3100C, LI-COR Biosciences, Lincoln, NE, USA). Leaf area index (LAI) was estimated by multiplying average leaf area by the number of individuals in a macrocosm, divided by ground area. LAI was 7.5 m2 m−2 in bean and 4.5 m2 m−2 in cotton. Though this was much higher than seen in field settings, a higher LAI was desirable because it leads to a higher proportion of shaded leaves, which in turn, should decrease the relative effect of circadian regulation over canopy scales. This will be discussed in detail later.
Leaf gas exchange was measured using a portable photosynthesis system (LI-6400XT, Li-Cor, Lincoln, Nebraska, USA), after setting the leaf cuvette to the same environmental conditions as the macrocosms. We conducted spot gas exchange measurements every 4 h in three leaves from the upper light-exposed part of the canopy within each macrocosm, and average values for each of the three macrocosms per species were used in subsequent analyses. Different leaves from different individuals were measured during each round of measurement. Leaf temperature was independently measured at the time of gas exchange measurements using an infrared thermometer (MS LT, Optris GmbH, Berlin, Germany). No significant difference with air temperature was recorded by the Tair probe (PC33, Mitchell Instrument SAS, Lyon, France) (intercept = −4.3 ± 4.5 [mean ± 95 % CI]; slope = 1.15 ± 0.17; R2 = 0.89).
Question 1: Does circadian regulation scale up to affect whole canopy fluxes?
Quantification of the circadian-driven range in variation of diurnal gas exchange
Variation during entrainment
Variation during constant conditions
% clock-driven variation
Leaf (μmol m−2 s−1)
Ecosystem (μmol m−2 s−1)
Leaf (μmol m−2 s−1)
Ecosystem (μmol m−2 s−1)
Leaf (conductance, mol m−2 s−1)
Ecosystem (l h−1)
Leaf (conductance, mol m−2 s−1)
Ecosystem (l h−1)
We examined statistical significance of these temporal patterns, and calculated the diurnal variation range under constant and changing environmental conditions, using a generalized additive mixed model (GAMM) fitted with automated smoothness selection  in the R software environment (mgcv library in R 3.1.2, R Foundation for Statistical Computing, Vienna, Austria), including macrocosms as a random factor. This approach makes no a priori assumption about the functional relationship between variables. We accounted for temporal autocorrelation in the residuals by adding a first-order autoregressive process structure (nlme library ). Significant temporal variation in the GAMM best-fit line was analyzed after computation of the first derivative (the slope, or rate of change) with the finite differences method. We also computed standard errors (SE) and a 95 % point-wise confidence interval for the first derivative. The trend was subsequently deemed significant when the derivative confidence interval was bounded away from zero at the 95 % level (for full details on this method see ). Non-significant periods, reflecting lack of local statistically significant trending, are illustrated on the figures by the yellow line portions, and significant differences occur elsewhere. The magnitude of the range in variation driven by the circadian clock (Table 1) was calculated using GAMM maximum and minimum predicted values.
In terms of the magnitude of the oscillation, there were some differences across species but, overall, similar patterns were observed (Fig. 1, Table 1). Circadian regulation was diluted as we moved up in scale. For instance, the magnitude of the clock-driven variation was 41/54 % of the total diurnal oscillation during entrainment for Al (bean/cotton), but 20/38 % for Ac. Similarly, while gs varied by 72/79 % under constant conditions, variation in Ec was 28/64 %. However, despite this dilution, we always observed a significant self-sustained 24-h oscillation in Ac as well as in Ec.
It could be argued that calculating the magnitude of circadian regulation tends to overestimate its importance because it is based upon a 24-h cycle, whereas in reality, no Ac occurs during the night, and Ec will be lower during a normal night (when it is dark) than in the ‘subjective’ night (the period under constant conditions when it would have been night-time during entrainment). Hence, we re-calculated the magnitude of the oscillation in Ac and Ec only during the 12 h of the subjective day under constant conditions and observed that it was 15.4 % and 24.0 %, respectively, for bean, and 29.75 % and 37.7 %, respectively, for cotton.
Question 2: Does adding a circadian oscillator improve the performance of stomatal models?
Three different model runs were conducted for each of the three different stomatal conductance models. First, each gs model was calibrated and validated with the entire leaf-level data set (Fig. 1). Second, we calibrated each model under changing diurnal conditions of PAR, Tair and VPD (first 24 h in Fig. 1) and validated it with data under constant PAR, Tair and VPD conditions (last 48 h in Fig. 1). Third, we calibrated each model under constant PAR, Tair and VPD conditions, and validated it with data under changing PAR, Tair and VPD. Given the distinctly different patterns of environmental conditions during the changing and constant phases, the last two model runs were included to represent changes in model fit under ‘novel’ environmental conditions. Importantly, the third model run was comparable with previous studies on the importance of circadian regulation for modeling in the field  because it used data collected under constant environmental conditions to infer the effect on gs over changing environmental conditions.
We observed that, in the absence of fluctuations in environmental drivers, both A and E statistically significant oscillations through time. According to conventional wisdom, diurnal variation during the entrainment phases is largely attributed to direct environmental effects of PAR, Tair and VPD on physiological processes [5, 6, 33–35]. However, our experiment using constant environmental conditions as a ‘control’ indicates that 20–79 % of the diurnal range in canopy CO2 and H2O fluxes can be recreated fully independently of environmental change (Fig. 1, Table 1). There are myriad endogenous processes that could affect diurnal carbon and water flux patterns, including carbohydrate accumulation  or hydraulic feedbacks . However, these feedbacks generally tend towards monotonically decreasing A and E over time. Instead, we observed that diurnal variation under a constant environment showed a period of ~24 h, which is consistent with a circadian gas exchange regulation [12, 37]. Furthermore, we observed that current stomatal models have a high predictive power, but the addition of circadian oscillators led to significant improvements in modeling outputs.
Circadian regulation of gas exchange from leaves to canopies
It is well known that radiation is the major environmental driver of gas exchange, and can create 100 % of the diurnal oscillation. Tair and VPD are often considered to be the next most important environmental drivers of diurnal flux dynamics. Although we did not measure the response of A or E to either Tair or VPD during these experiments, other studies with these species typically document that, in the absence of strong environmental stress, Tair and VPD lead to diurnal flux variation of the same order of magnitude as those observed in this study . In other words, the circadian oscillation in Ac and Ec observed in this study (Table 1) could be comparable to that documented in Tair or VPD response curves.
To fully understand the upscaling of circadian rhythms, further exploration is required into how canopy structure determines the canopy-level expression of circadian regulation. Circadian regulation in understory species has been shown to be less important than in overstory species . We hypothesize that this might occur because the predictability and variability of environmental cues diminish under a canopy. We therefore expect a higher dilution of circadian regulation across scales in forests with highly structured multilayer canopies, where a relatively larger proportion of carbon fixation and water loss may be conducted by shaded leaves, than in forests or grasslands with a single layer canopy. This hypothesis could explain why we always observe a higher degree of circadian-driven variation at the leaf-level compared to canopy-level fluxes (Table 1). Additionally, Aschoff’s rules would predict a lengthening of circadian periodicity under low irradiances, which would additionally contribute towards the apparent decrease in amplitude of the circadian rhythm when integrated over the whole canopy . Indeed, there was a higher dilution of circadian regulation at the canopy-level in bean compared to cotton; i.e., the difference between the range of the circadian-driven oscillation between Al and Ac, and also between gs and Ec, is always higher in bean than in cotton (Table 1). This could be explained by our measurements of a higher LAI, and therefore a higher proportion of shaded leaves, in bean (7.5 m2 m−2) than in cotton (4.5 m2 m−2). By measuring only leaf-level gas exchange in the light-exposed upper part of the canopy, we did not capture the reduced rhythms in carbon fixation in the shaded lower part of the canopy; hence the observed dilution across scales is likely the result of not documenting gas exchange in these shaded leaves. Greater understanding of the relative importance of circadian regulation on ecosystem processes, as a function of leaf canopy structure, should be a future research objective. Yet, it is remarkable that despite high LAI in our study, circadian regulation was still apparent at the canopy level.
Modeling circadian regulation of gas exchange
Similar to previous approaches , we conducted a modeling exercise where, among others, gs was calibrated with the constant conditions data set, and then validated under changing conditions. Although validation did not occur under strictly field conditions, they were field-like. Hence, the significant improvements in model fits observed when including a circadian oscillator, lead us to conclude that the assertion of circadian rhythms having insignificant rhythms for gas exchange under field settings needs to be revised.
Circadian regulation had a more important effect on stomatal conductance and ecosystem transpiration than on leaf and canopy carbon assimilation (Fig. 1). This may explain why circadian regulation significantly improved stomatal model output in our study, but not in previous studies on photosynthesis . It is worth noting the many reports of hysteresis in tree transpiration in which, for a given environmental condition, transpiration was found to be higher in the morning than in the afternoon [40–42]. This phenomenon has often been explained in terms of hydraulic feedbacks on stomata. However, our results, along with further experiments on circadian regulation of stomata [8, 43], indicate that circadian rhythmicity could at least partly explain hysteretic water fluxes.
Our empirical approach considers time as a surrogate of circadian regulation. Importantly, we observed that the circadian oscillator enhanced the performance of diurnal leaf-level stomatal models (Table 2). We acknowledge that the use of time as a surrogate for circadian action is not fully satisfactory; yet, given present limited understanding of circadian processes at the scale of relevance for this analysis, this is the only approach available.
Previous studies have shown the clock to regulate gs independently from Al [44, 45]; i.e., the circadian pattern in leaf carbon assimilation is a function of circadian regulation of leaf biochemistry, and independent of variation in stomatal conductance [19, 44, 46]. In this study, our goal was not to assess the mechanisms driving circadian rhythms in stomata and photosynthesis. However, we note that mechanisms underlying circadian gas exchange regulation are mostly studied at molecular or cellular scales. Focusing on the mechanisms underlying circadian regulation, at the scales relevant for ecosystem studies, should be at the forefront of future research efforts.
To determine the impact of circadian regulation on gas exchange, additional studies across phylogenies and functional groups are required. Although current evidence suggests a highly conserved genetic make-up of circadian rhythm in plants , the suite of environmental conditions interacting with circadian regulation of gas exchange remains unknown . Similarly, although our study was performed at much higher PAR levels than in growth chambers (usually < 200 μmol m−2 s−1), where the circadian clock is most often assessed, PAR was less than full sun and below photosynthetic saturation levels. Additional experiments should address the role of circadian regulation under saturating radiation.
Our results contribute to the expanding field of plant ‘memory’, in that the circadian clock regulates gas exchange based upon the conditions of the previous days. Conceptual frameworks on the effects of ‘memory’ on ecological systems often consider the effect of legacies from antecedent environmental stress , and potential epigenetic regulation . Circadian regulation could act as an adaptive memory whereby a plant’s metabolism is adjusted based on the conditions experienced in previous days, and fitness is increased via anticipation  and growth regulation [51, 52]. Additional studies are required to expand current frameworks on how to incorporate memory from ecological processes into global change models.
The experiment was performed at the Macrocosms platform of the Montpellier European Ecotron, Centre National de la Recherche Scientifique (CNRS, France). Twelve outdoor macrocosms (six planted with bean and six with cotton) were used, where the main abiotic drivers (air temperature, humidity and CO2 concentration) were automatically controlled. In each macrocosm, plants were grown on soil (area of 2 m2, depth of 2 m) contained in a lysimeter resting on a weighing platform. Soil was collected from the flood plain of the Saale River near Jena, Germany, and used in a previous Ecotron experiment on biodiversity . After that experiment, the soil was ploughed down to 40 cm and fertilized with 25/25/35 NPK (MgO, SO3 and other oligo-elements were associated in this fertilizer: Engrais bleu universel, BINOR, Fleury-les-Aubrais, FR).
The soil was regularly watered to ca field capacity by drip irrigation, although irrigation was stopped during each measurement campaign (every few days) to avoid interference with water flux measurements. However, no significant differences (at P < 0.05, paired t-test, n = 3) in leaf water potential occurred between the beginning and end of these measurement campaigns, indicating no effect of a potentially declining soil moisture on leaf hydration .
Environmental conditions within the macrocosms (excluding the experimental periods) were set to mimic outdoor conditions, but did include a 10 % light reduction by the macrocosm dome cover. During experimental periods, light was controlled by placing a completely opaque fitted cover on each dome to block external light inputs (polyvinyl chloride-coated polyester sheet Ferrari 502, assembled by IASO, Lleida, Spain), and by using a set of five dimmable plasma lamps (GAN 300 LEP with the Luxim STA 4102 bulb, with a sun-like light spectrum, see Additional file 2); these lamps were hung 30 cm above the plant canopy and provided a PAR of 500 μmol m−2 s−1 (the maximum possible with these lamps). PAR was measured at the canopy level with a quantum sensor (Li-190, LI-COR Biosciences, Lincoln, NE, USA) in each macrocosm and corroborated a continuous PAR of 500 μmol m−2 s−1 during the experiment.
Bean and cotton were planted in five different rows within the domes on 10th July 2013, one month before the start of the measurements, and thinned to densities of 105 and 9 individuals per square meter, respectively. Cotton (STAM-A16 variety, Institut National de Recherche Agronomique du Bénin (INRAB) / Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)) is a perennial shrub with an indeterminate growth habit. This cotton variety grows to 1.5–2 m tall and has a pyramidal shape and short branches. Bean is an annual herbaceous species. Recombinant inbred line (RIL)-115 (bred by the Institut National de la Recherche Agronomique (INRA) ‘Eco & Sols’) was used, which is a fast growing, indeterminate dwarf variety, growing 0.3–0.5 m tall. Bean plants were inoculated with Rhizobium tropici CIAT 899, also provided by INRA. During the experiment, bean and cotton generally remained at the inflorescence emergence developmental growth stage (, codes 51–59 in the BBCH scale, the standard phenological scale within the crop industry; ). No specific license or permission was required.
- A c :
Canopy net CO2 exchange
Akaike information criterion
- A l :
Leaf level net photosynthesis
Centre de Coopération Internationale en Recherche Agronomique pur le Développement (Center for International Cooperation in Agronomic Research for Development)
Centre National de la Recherche Scientifique (French National Center for Scientific Research)
- E c :
Generalized additive mixed model
- g s :
Institut National de la Recherche Agronomique (French National Institute for Agronomic Research)
Institut National de la Recherche Agronomique du Bénin (Benin National Institute for Agronomic Research)
Leaf area index
Photosynthetically active radiation
Recombinant inbred line
- T air :
Vapor pressure deficit.
We remain indebted to Edward Gerardeaux, Dominique Dessauw, Janine Jean, Patrick Prudent (Aïda CIRAD), Jean-Jacques Drevon, Catherine Pernot (INRA Eco & Sols), Bruno Buatois, Alain Rocheteau (Centre d’Ecologie Fontionnelle et Evolutive, CNRS), Alex Pra, Amal Mokhtar, and the full Ecotron team, in particular Christophe Escape, for outstanding technical assistance during experimental set-up, plant cultivation, or subsequent measurements.
This study benefited from the CNRS human and technical resources allocated to the Research Infrastructure Ecotrons, as well as from the state allocation ‘Investissement d'Avenir’ ANR-11-INBS-0001; ExpeER Transnational Access program; Ramón y Cajal fellowships (RYC-2012-10970 to VRD and RYC-2008-02050 to JPF); the Erasmus Mundus Master Course Mediterranean Forestry and Natural Resources Management (MEDfOR); and internal grants from the Leibniz Centre for Agricultural Landscape Research to AG, and from the Western Sydney University’s Hawkesbury Institute for the Environment and the Spanish Government (AGL2015-69151-R) to VRD.
Availability of data and materials
All supporting data and materials for this research paper are hosted in the GigaDB repository .
VRD, AG and JR designed the research with input from JPF, MB, AM, DTT, and MGT. JR, SD, DL, CP, SS, JPF, OR, VRD, AG, JGA, JC, SGM, ZK, PM, AM, KPW, and JV performed the research. VRD and JGA analyzed the data. VRD coordinated the writing of the manuscript. The manuscript was written by VRD, MB, MGT, JR, AG, JPF, DTT, JV, AM and JGA, with substantial contributions from JC, SGM, ZK, PM and KPW. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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